《植物生理学报》 2018, 54(1): 81-91
通信作者：蒙美莲；E-mail: mmeilian@126.com
摘 要：
为研究干旱胁迫及复水对马铃薯(Solanum tuberosum)类黄酮合成途径影响的机制, 以‘克新1号’为试验材料, 利用高通量测序技术分析了苗期干旱处理(40%土壤相对含水量维持14 d, DT)与对照(70%土壤相对含水量维持14 d, DCK)、复水处理(40%土壤相对含水量持续14 d, 然后恢复到70%土壤相对含水量维持7 d, RT)与对照(70%土壤相对含水量维持21 d, RCK)之间类黄酮合成途径中基因表达的差异。结果表明, 干旱胁迫后, 马铃薯中类黄酮合成途径变化显著(P<0.01), 该途径共有10个基因发生了差异表达, 影响7个酶的合成, 其中查尔酮合酶(EC 2.3.1.74)、柚皮素3-双加氧酶(EC 1.14.11.9)、类黄酮3′,5′-羟化酶(EC 1.14.13.88)的基因表达显著上调, 从而可能促进了异黄酮、黄酮醇和黄酮以及高圣草素、二氢杨梅素的形成。PGSC0003DMG400019110和PGSC0003DMG400003563的上调表达导致查尔酮合酶和柚皮素3-双加氧酶的合成加快。复水对类黄酮合成途径的恢复效果较好, 差异表达基因减少到2个, 除莽草酸邻羟基肉桂酰转移酶(EC 2.3.1.133)与咖啡酰CoA-O-甲基转移酶(EC 2.1.1.104)的基因表达仍存在显著差异外, 其余酶均已无差异。关键词：马铃薯; 干旱胁迫; 复水; 类黄酮; 查尔酮合酶
收稿：2017-05-22 修定：2017-12-20
资助：国家现代农业产业技术体系建设(CARS-10-P17)。
Corresponding author: MENG Mei-Lian; E-mail: mmeilian@126.com
Abstract:
In order to understand the influence of drought stress and rehydration on the synthesis and regulation of flavonoids in potato (Solanum tuberosum), differential gene expression of flavonoid synthesis was investigated in this study. Using cultivar ‘Kexin No.1’ as experimental material, high-throughput sequencing technique was used. The experimental treatments included drought treatment (40% relative soil water content for 14 d, DT) and its control (70% relative soil water content for 14 d, DCK), rehydration treatment (40% relative soil water content for 14 d and then rehydrated to 70% for 7 d, RT) and its control (70% relative soil water content for 21 d, RCK). The results showed that flavonoids biosynthesis in potato was significantly regulated by drought stress (P<0.01). Altogether 10 genes in the pathway were differentially expressed between DT and DCK treatments, which could affect the activities of seven enzymes. The genes encoding chalcone synthase (EC 2.3.1.74), naringenin-3-dioxygenase (EC 1.14.11.9) and flavonoid 3',5'-hydroxylase (EC 1.14.13.88) were upregulated significantly under drought stress, which might further affect the formation of isoflavones, flavonols, flavonoids, homoeriodictyol and dihydromyricetin. The upregulation of PGSC0003DMG400019110 and PGSC0003DMG400003563 promoted the biogenesis of chalcone synthase and naringenin-3-dioxygenase. Rehydration after drought stress restored flavonoid biosynthesis to an extent, the number of differentially expressed genes reduced from 10 to 2, only genes encoding shikimate O-hydroxycinnamoyl transferase (EC 2.3.1.133) and caffeoyl coenzyme A O-methyltransferase (EC 2.1.1.104) were differentially expressed.Key words: potato; drought stress; rehydration; flavonoid; chalcone synthase
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